Substrate Incorporation Modes in Aminopeptidase Mimics
نویسندگان
چکیده
منابع مشابه
Aminopeptidase P: cation activation and inhibitor sensitivity are substrate-dependent.
Aminopeptidase P (AP-P, EC 3.4.1 1.9) was first identified in pig kidney as an cxopeptidase capable of cleaving the Nterminal amino acid residue from peptides with a penultimate proline. Peptidcs of the X-Pro-Y type were cleaved most readily, and a synthetic substrate, glycyl-prolyl-hydroxyproline (Gly-Pro-Hyp), was originally used to monitor AP-P activity [ I ] . The enzyme has sincc been show...
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Chemical imaging techniques have played instrumental roles in dissecting the spatiotemporal regulation of signal transduction pathways. Phospholipase D (PLD) enzymes affect cell signaling by producing the pleiotropic lipid second messenger phosphatidic acid via hydrolysis of phosphatidylcholine. It remains a mystery how this one lipid signal can cause such diverse physiological and pathological...
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Zhun-Yong Ong1,2,* and Eric Pop1,3,4,† 1Micro and Nanotechnology Laboratory, University of Illinois, Urbana-Champaign, Illinois 61801, USA 2Department of Physics, University of Illinois, Urbana-Champaign, Illinois 61801, USA 3Department of Electrical and Computer Engineering, University of Illinois, Urbana-Champaign, Illinois 61801, USA 4Beckman Institute, University of Illinois, Urbana-Champai...
متن کاملExcretion of X-prolyl dipeptidyl-aminopeptidase in human urine as determined with a new fluorogenic substrate.
X-Prolyl dipeptidyl-aminopeptidase activity was found in human urine by a sensitive fluorescence assay in which a new fluorogenic substrate, 7-glycylproline-4-methylcoumarinamide, is used. The Km value was 2.9 X 10(-4) mol/liter, and the optimum pH was 8.7 in glycine-NaOH buffer. The enzyme activity was stable at 4 degrees C for at least five days. On Sephadex G-200 column chromatography, norma...
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ژورنال
عنوان ژورنال: Journal of Computer Chemistry, Japan
سال: 2014
ISSN: 1347-1767,1347-3824
DOI: 10.2477/jccj.2013-0028